食用菌种植 草菇种植 百欧博伟生物Hepa1-6[Hepa1-6]ATCC细胞库 免费发布信息

百欧博伟生物Hepa1-6[Hepa1-6]ATCC细胞库

2018-12-21 编号:248388659
1
  • 中国微生物菌种查询网
  • Hepa 1-6,百欧博伟生物,ATCC细胞库
  • 李果
  • 18610886153
  • 010-51291224
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产品详情

产品名Hepa 1-6,百欧博伟生物,ATCC细胞库
面向地区全国
品牌中国微生物菌种查询网
产地美国
品种纯度其它
染菌率%
出菇适温
水分≤其它
百欧博伟生物Hepa1-6[Hepa1-6]ATCC细胞库
百欧博伟生物Hepa1-6[Hepa1-6]ATCC细胞库
中国微生物菌种查询网隶属于北京百欧博伟生物技术有限公司,成立于二〇一四年十月份,是一家专业提供中国微生物菌种,标准菌种,质控菌株,ATCC菌种,细胞系以及培养基等产品的查询网站!自成立至今,已提供各类微生物菌种、细胞、培养基数量达四万余份,接待咨询客户数量5万余人次,成交合同数量2万余份!是国内微生物菌种及细胞信息最全的网站! 我们拥有自主研发能力,提供的ATCC菌种达15000余份,ATCC各类细胞系达3000余份,保藏中心涵盖菌种资源更达数十万余份!
Hepa 1-6 [Hepa1-6]
(ATCC? CRL-1830?)
Organism Mus musculus, mouse
Tissue liver
Product Format frozen Morphology epithelial Culture Properties adherent
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease hepatoma
Strain C57L
Applications 用途 This line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phaseDerivation This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Genes Expressed albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase
Cellular Products albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase
Comments 注释 Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
1,Remove and discard culture medium.
2,Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3,Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37℃ to facilitate dispersal.
4,Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5,Add appropriate aliquots of the cell suspension to new culture vessels.
6,Incubate cultures at 37℃.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Two to three times weekly
Cryopreservation Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃
Growth Conditions: The cells can also be propagated in a serum free medium consisting of 3 parts Dulbecco's modified Eagle's medium, 1 part Waymouth's MAB 87/3 medium and 30 nM selenium.

北京百欧博伟生物技术有限公司提供百欧博伟生物Hepa1-6[Hepa1-6]ATCC细胞库,包括Hepa 1-6,百欧博伟生物,ATCC细胞库的详细产品价格、产品图片等产品介绍信息。

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